James Arnold Taylor

Leonardo (voice)

Mitchell Whitfield

Donatello / Additional Voices (voice)

Nolan North

Raphael / Nightwatcher (voice)

Mikey Kelley

Michelangelo (voice)

Sarah Michelle Gellar

April O'Neil (voice)

Chris Evans

Casey Jones (voice)

Mako

Splinter (voice)

Patrick Stewart

Winters (voice)

Kevin Smith

Diner Cook (voice)

Laurence Fishburne

Narrator (voice)

Zhang Ziyi

Karai (voice)

John DiMaggio

Colonel Santino (voice)

Paula Mattioli

General Serpiente (voice)

Kevin Michael Richardson

General Aguila / Additional Voices (voice)

Fred Tatasciore

General Gato / Additional Voices (voice)

Dee Bradley Baker

Additional Voices (voice)

Greg Baldwin

Splinter (voice)

Jeff Bennett

Additional Voices (voice)

Jim Cummings

Additional Voices (voice)

Grey DeLisle

Additional Voices (voice)

Chris Edgerly

Additional Voices (voice)

Kim Mai Guest

Additional Voices (voice)

Jennifer Hale

Additional Voices (voice)

Jess Harnell

Additional Voices (voice)

Phil LaMarr

Additional Voices (voice)

Paul Michael Robinson

Additional Voices (voice)

Tara Strong

Additional Voices (voice)

Billy West

Additional Voices (voice)

Kevin Munroe

Diner Patron (voice) (uncredited)